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Image Search Results
Journal: bioRxiv
Article Title: Rhodococcus parequi sp. nov., a new species isolated from equine farm soil closely related to the pathogen Rhodococcus equi
doi: 10.1101/2024.12.09.627583
Figure Lengend Snippet: Heat map of Phenotype MicroArray TM (PM) results for carbon and nitrogen source utilization by PAM 2766 T , R. equi DSM 20307 T and R. equi 103S (PAM 1126). Bacterial inocula were grown at 30°C in TSB until the stationary phase and then suspended in mReMM mineral medium and transferred to the PM plates. Incubabion was performed at 30°C with OD 590 monitored every 15 min for 48 hours in an OmniLog reader. Strains were tested in duplicate and results were analysed using OmniLog software. Maximum growth is represented in graded colours from lowest (black) to highest (yellow). Red arrows indicate differential utilization of a substrate between PAM 2766 T and R. equi. Black arrows in the PM1 and PM2A plates indicate a carbon source utilized by the three tested bacteria (see Table S2 for detailed results). Asterisks indicate false positive reactions in the PM2A plate previously reported in ref. .
Article Snippet:
Techniques: Microarray, Software, Bacteria
Journal: Cell
Article Title: A white-box machine learning approach for revealing antibiotic mechanisms of action
doi: 10.1016/j.cell.2019.04.016
Figure Lengend Snippet: (A) Overall experimental design for measuring metabolite effects on antibiotic lethality. Overnight cultures of E. coli MG1655 were inoculated into MOPS minimal medium, grown to early exponential phase, and back-diluted to OD600 = 0.1. Cells were dispensed into Biolog phenotype microarray plates (PMs) 1–4 (Bochner, 2009) with different concentrations of ampicillin (AMP), ciprofloxacin (CIP) or gentamicin (GENT) added. OD600 was measured after 4 hours of incubation at 37°C and 900 rpm shaking. Antibiotic IC50s were estimated for each antibiotic-metabolite combination.
Article Snippet: Cells were dispensed into
Techniques: Microarray, Incubation
Journal: Cell
Article Title: A white-box machine learning approach for revealing antibiotic mechanisms of action
doi: 10.1016/j.cell.2019.04.016
Figure Lengend Snippet: Key Resources Table
Article Snippet: Cells were dispensed into
Techniques: Virus, Recombinant, Microarray, Software, Combined Bisulfite Restriction Analysis Assay