biolog phenotypic microarray competitive index Search Results


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Biolog Inc phenotype microarray tm
Heat map of Phenotype <t>MicroArray</t> TM (PM) results for carbon and nitrogen source utilization by PAM 2766 T , R. equi DSM 20307 T and R. equi 103S (PAM 1126). Bacterial inocula were grown at 30°C in TSB until the stationary phase and then suspended in mReMM mineral medium and transferred to the PM plates. Incubabion was performed at 30°C with OD 590 monitored every 15 min for 48 hours in an OmniLog reader. Strains were tested in duplicate and results were analysed using OmniLog software. Maximum growth is represented in graded colours from lowest (black) to highest (yellow). Red arrows indicate differential utilization of a substrate between PAM 2766 T and R. equi. Black arrows in the PM1 and PM2A plates indicate a carbon source utilized by the three tested bacteria (see Table S2 for detailed results). Asterisks indicate false positive reactions in the PM2A plate previously reported in ref. .
Phenotype Microarray Tm, supplied by Biolog Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Biolog Inc biolog phenotype microarray plates (pms) 1–4
(A) Overall experimental design for measuring metabolite effects on antibiotic lethality. Overnight cultures of E. coli MG1655 were inoculated into MOPS minimal medium, grown to early exponential phase, and back-diluted to OD600 = 0.1. Cells were dispensed into Biolog phenotype <t>microarray</t> plates (PMs) 1–4 (Bochner, 2009) with different concentrations of ampicillin (AMP), ciprofloxacin (CIP) or gentamicin (GENT) added. OD600 was measured after 4 hours of incubation at 37°C and 900 rpm shaking. Antibiotic IC50s were estimated for each antibiotic-metabolite combination.
Biolog Phenotype Microarray Plates (Pms) 1–4, supplied by Biolog Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Biolog Inc phenotype biolog microarrays plates pm02a
(A) Overall experimental design for measuring metabolite effects on antibiotic lethality. Overnight cultures of E. coli MG1655 were inoculated into MOPS minimal medium, grown to early exponential phase, and back-diluted to OD600 = 0.1. Cells were dispensed into Biolog phenotype <t>microarray</t> plates (PMs) 1–4 (Bochner, 2009) with different concentrations of ampicillin (AMP), ciprofloxacin (CIP) or gentamicin (GENT) added. OD600 was measured after 4 hours of incubation at 37°C and 900 rpm shaking. Antibiotic IC50s were estimated for each antibiotic-metabolite combination.
Phenotype Biolog Microarrays Plates Pm02a, supplied by Biolog Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Biolog Inc biolog microarray
(A) Overall experimental design for measuring metabolite effects on antibiotic lethality. Overnight cultures of E. coli MG1655 were inoculated into MOPS minimal medium, grown to early exponential phase, and back-diluted to OD600 = 0.1. Cells were dispensed into Biolog phenotype <t>microarray</t> plates (PMs) 1–4 (Bochner, 2009) with different concentrations of ampicillin (AMP), ciprofloxacin (CIP) or gentamicin (GENT) added. OD600 was measured after 4 hours of incubation at 37°C and 900 rpm shaking. Antibiotic IC50s were estimated for each antibiotic-metabolite combination.
Biolog Microarray, supplied by Biolog Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Biolog Inc 96-well phenotype microarray plates pm1-pm5
(A) Overall experimental design for measuring metabolite effects on antibiotic lethality. Overnight cultures of E. coli MG1655 were inoculated into MOPS minimal medium, grown to early exponential phase, and back-diluted to OD600 = 0.1. Cells were dispensed into Biolog phenotype <t>microarray</t> plates (PMs) 1–4 (Bochner, 2009) with different concentrations of ampicillin (AMP), ciprofloxacin (CIP) or gentamicin (GENT) added. OD600 was measured after 4 hours of incubation at 37°C and 900 rpm shaking. Antibiotic IC50s were estimated for each antibiotic-metabolite combination.
96 Well Phenotype Microarray Plates Pm1 Pm5, supplied by Biolog Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Biolog Inc biolog phenotyping microarrays
(A) Overall experimental design for measuring metabolite effects on antibiotic lethality. Overnight cultures of E. coli MG1655 were inoculated into MOPS minimal medium, grown to early exponential phase, and back-diluted to OD600 = 0.1. Cells were dispensed into Biolog phenotype <t>microarray</t> plates (PMs) 1–4 (Bochner, 2009) with different concentrations of ampicillin (AMP), ciprofloxacin (CIP) or gentamicin (GENT) added. OD600 was measured after 4 hours of incubation at 37°C and 900 rpm shaking. Antibiotic IC50s were estimated for each antibiotic-metabolite combination.
Biolog Phenotyping Microarrays, supplied by Biolog Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Biolog Inc metabolic phenotype microarray-mammalian (pm-m) cell assays
(A) Overall experimental design for measuring metabolite effects on antibiotic lethality. Overnight cultures of E. coli MG1655 were inoculated into MOPS minimal medium, grown to early exponential phase, and back-diluted to OD600 = 0.1. Cells were dispensed into Biolog phenotype <t>microarray</t> plates (PMs) 1–4 (Bochner, 2009) with different concentrations of ampicillin (AMP), ciprofloxacin (CIP) or gentamicin (GENT) added. OD600 was measured after 4 hours of incubation at 37°C and 900 rpm shaking. Antibiotic IC50s were estimated for each antibiotic-metabolite combination.
Metabolic Phenotype Microarray Mammalian (Pm M) Cell Assays, supplied by Biolog Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Biolog Inc omnilog phenotyping microarray (pm) system
(A) Overall experimental design for measuring metabolite effects on antibiotic lethality. Overnight cultures of E. coli MG1655 were inoculated into MOPS minimal medium, grown to early exponential phase, and back-diluted to OD600 = 0.1. Cells were dispensed into Biolog phenotype <t>microarray</t> plates (PMs) 1–4 (Bochner, 2009) with different concentrations of ampicillin (AMP), ciprofloxacin (CIP) or gentamicin (GENT) added. OD600 was measured after 4 hours of incubation at 37°C and 900 rpm shaking. Antibiotic IC50s were estimated for each antibiotic-metabolite combination.
Omnilog Phenotyping Microarray (Pm) System, supplied by Biolog Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Biolog Inc phenotype microarray (pm) panels 1–20
(A) Overall experimental design for measuring metabolite effects on antibiotic lethality. Overnight cultures of E. coli MG1655 were inoculated into MOPS minimal medium, grown to early exponential phase, and back-diluted to OD600 = 0.1. Cells were dispensed into Biolog phenotype <t>microarray</t> plates (PMs) 1–4 (Bochner, 2009) with different concentrations of ampicillin (AMP), ciprofloxacin (CIP) or gentamicin (GENT) added. OD600 was measured after 4 hours of incubation at 37°C and 900 rpm shaking. Antibiotic IC50s were estimated for each antibiotic-metabolite combination.
Phenotype Microarray (Pm) Panels 1–20, supplied by Biolog Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Biolog Inc biolog phenotype microarray analysis software
(A) Overall experimental design for measuring metabolite effects on antibiotic lethality. Overnight cultures of E. coli MG1655 were inoculated into MOPS minimal medium, grown to early exponential phase, and back-diluted to OD600 = 0.1. Cells were dispensed into Biolog phenotype <t>microarray</t> plates (PMs) 1–4 (Bochner, 2009) with different concentrations of ampicillin (AMP), ciprofloxacin (CIP) or gentamicin (GENT) added. OD600 was measured after 4 hours of incubation at 37°C and 900 rpm shaking. Antibiotic IC50s were estimated for each antibiotic-metabolite combination.
Biolog Phenotype Microarray Analysis Software, supplied by Biolog Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 90 stars, based on 1 article reviews
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Biolog Inc phenotype microarray carbon microplate pm1
(A) Overall experimental design for measuring metabolite effects on antibiotic lethality. Overnight cultures of E. coli MG1655 were inoculated into MOPS minimal medium, grown to early exponential phase, and back-diluted to OD600 = 0.1. Cells were dispensed into Biolog phenotype <t>microarray</t> plates (PMs) 1–4 (Bochner, 2009) with different concentrations of ampicillin (AMP), ciprofloxacin (CIP) or gentamicin (GENT) added. OD600 was measured after 4 hours of incubation at 37°C and 900 rpm shaking. Antibiotic IC50s were estimated for each antibiotic-metabolite combination.
Phenotype Microarray Carbon Microplate Pm1, supplied by Biolog Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Biolog Inc tetrazolium-based redox assay biolog phenotype microarrays
(A) Overall experimental design for measuring metabolite effects on antibiotic lethality. Overnight cultures of E. coli MG1655 were inoculated into MOPS minimal medium, grown to early exponential phase, and back-diluted to OD600 = 0.1. Cells were dispensed into Biolog phenotype <t>microarray</t> plates (PMs) 1–4 (Bochner, 2009) with different concentrations of ampicillin (AMP), ciprofloxacin (CIP) or gentamicin (GENT) added. OD600 was measured after 4 hours of incubation at 37°C and 900 rpm shaking. Antibiotic IC50s were estimated for each antibiotic-metabolite combination.
Tetrazolium Based Redox Assay Biolog Phenotype Microarrays, supplied by Biolog Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Image Search Results


Heat map of Phenotype MicroArray TM (PM) results for carbon and nitrogen source utilization by PAM 2766 T , R. equi DSM 20307 T and R. equi 103S (PAM 1126). Bacterial inocula were grown at 30°C in TSB until the stationary phase and then suspended in mReMM mineral medium and transferred to the PM plates. Incubabion was performed at 30°C with OD 590 monitored every 15 min for 48 hours in an OmniLog reader. Strains were tested in duplicate and results were analysed using OmniLog software. Maximum growth is represented in graded colours from lowest (black) to highest (yellow). Red arrows indicate differential utilization of a substrate between PAM 2766 T and R. equi. Black arrows in the PM1 and PM2A plates indicate a carbon source utilized by the three tested bacteria (see Table S2 for detailed results). Asterisks indicate false positive reactions in the PM2A plate previously reported in ref. .

Journal: bioRxiv

Article Title: Rhodococcus parequi sp. nov., a new species isolated from equine farm soil closely related to the pathogen Rhodococcus equi

doi: 10.1101/2024.12.09.627583

Figure Lengend Snippet: Heat map of Phenotype MicroArray TM (PM) results for carbon and nitrogen source utilization by PAM 2766 T , R. equi DSM 20307 T and R. equi 103S (PAM 1126). Bacterial inocula were grown at 30°C in TSB until the stationary phase and then suspended in mReMM mineral medium and transferred to the PM plates. Incubabion was performed at 30°C with OD 590 monitored every 15 min for 48 hours in an OmniLog reader. Strains were tested in duplicate and results were analysed using OmniLog software. Maximum growth is represented in graded colours from lowest (black) to highest (yellow). Red arrows indicate differential utilization of a substrate between PAM 2766 T and R. equi. Black arrows in the PM1 and PM2A plates indicate a carbon source utilized by the three tested bacteria (see Table S2 for detailed results). Asterisks indicate false positive reactions in the PM2A plate previously reported in ref. .

Article Snippet: Phenotype MicroArray TM (Biolog Inc.) screens for carbon (PM1 and PM2A plates) and nitrogen (PM3B plates) sources were used to identify additional differential phenotypic markers.

Techniques: Microarray, Software, Bacteria

(A) Overall experimental design for measuring metabolite effects on antibiotic lethality. Overnight cultures of E. coli MG1655 were inoculated into MOPS minimal medium, grown to early exponential phase, and back-diluted to OD600 = 0.1. Cells were dispensed into Biolog phenotype microarray plates (PMs) 1–4 (Bochner, 2009) with different concentrations of ampicillin (AMP), ciprofloxacin (CIP) or gentamicin (GENT) added. OD600 was measured after 4 hours of incubation at 37°C and 900 rpm shaking. Antibiotic IC50s were estimated for each antibiotic-metabolite combination.

Journal: Cell

Article Title: A white-box machine learning approach for revealing antibiotic mechanisms of action

doi: 10.1016/j.cell.2019.04.016

Figure Lengend Snippet: (A) Overall experimental design for measuring metabolite effects on antibiotic lethality. Overnight cultures of E. coli MG1655 were inoculated into MOPS minimal medium, grown to early exponential phase, and back-diluted to OD600 = 0.1. Cells were dispensed into Biolog phenotype microarray plates (PMs) 1–4 (Bochner, 2009) with different concentrations of ampicillin (AMP), ciprofloxacin (CIP) or gentamicin (GENT) added. OD600 was measured after 4 hours of incubation at 37°C and 900 rpm shaking. Antibiotic IC50s were estimated for each antibiotic-metabolite combination.

Article Snippet: Cells were dispensed into Biolog phenotype microarray plates (PMs) 1–4 ( Bochner, 2009 ) with different concentrations of ampicillin (AMP), ciprofloxacin (CIP) or gentamicin (GENT) added.

Techniques: Microarray, Incubation

Key Resources Table

Journal: Cell

Article Title: A white-box machine learning approach for revealing antibiotic mechanisms of action

doi: 10.1016/j.cell.2019.04.016

Figure Lengend Snippet: Key Resources Table

Article Snippet: Cells were dispensed into Biolog phenotype microarray plates (PMs) 1–4 ( Bochner, 2009 ) with different concentrations of ampicillin (AMP), ciprofloxacin (CIP) or gentamicin (GENT) added.

Techniques: Virus, Recombinant, Microarray, Software, Combined Bisulfite Restriction Analysis Assay